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SDS-PAGE (Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis) separates proteins according to their primary structure of size but not amino acid sequence.

Properties of SDS-PAGE

  • Acrylamide pore size depends on concentration.
  • Small proteins travel quickly through pores.
  • Mobility is proportional to log(MW) (Molecular Weight)
  • Sodium Dodecyl Sulfate (SDS) disrupts non-covalent interactions, thus gives proteins a negative charge.
  • Beta-Mercaptoethanol disrupts disulphide bonds.
  • Quanternary Structure is H2L2 where H2 (High MW) and L2 (Low MW)